PRAME Knockout cell line (A375)

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location： Home > Products > KO Cell Lines > PRAME Knockout cell line (A375)

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PRAME Knockout cell line (A375) Order

PRAME Knockout cell line (A375)
Catalog#
YKO-H1073
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PRAME Knockout cell line (A375)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PRAME Knockout cell line (A375)
Species
Human
Gene Name
PRAME
Gene ID
23532
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript PRAME-205)
Validation: PCR+Sanger Sequencing
PRAME Knockout cell line (A375) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PRAME gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A375 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PRAME gene knockout PRAME Knockout cell line (A375) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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